TelN Protelomerase
Overview
Cuts dsDNA at a TelN recognition sequence (56 bp) and leaves covalently closed ends at the site of cleavage.
Highlights
Catalyzes dsDNA at a TelN recognition sequence (56 bp)
Leaves covalently closed ends at the site of cleavage
Product Source
Purified from a strain of E. coli that expresses TelN Protelomerase of phage N15
Product Usage
Supplied in
- 10 mM Tris-HCl
- 100 mM NaCl
- 0.1 mM EDTA
- 1 mM DTT
- 50% glycerol
- pH 7.4 @ 25°C
Reaction Conditions
- 1X ThermoPol® Reaction Buffer
- 20 mM Tris-HCl
- 10 mM (NH4)2SO4
- 10 mM KCl
- 2 mM MgSO4
- 0.1% Triton® X-100
- (pH 8.8 @ 25°C)
Inactivation
75°C for 5 minutes
Unit Definition
One unit is defined as the amount of enzyme required to cleave 0.5 μg of linearized control plasmid in a total reaction volume of 50 μl in 30 minutes at 30°C in 1X ThermoPol Reaction Buffer