Phi29 DNA Polymerase
Overview
Phi29 DNA Polymerase responsible for the replication of the Bacillus Subtilis phage φ29. The enzyme is a highly processive DNA polymerase (up to 70kb insertions) with exceptional strand displacement properties and a 3’→5’ proofreading exonuclease function.
Highlights
3’→5’ proofreading exonuclease-High fidelity
Extreme processivity-Generation of large fragments
Isothermal amplification-No need for a thermocycler
Product Source
A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29
Product Usage
Supplied in
10 mM Tris-HCl
100 mM NaCl
0.1 mM EDTA
1 mM DTT
50% glycerol
pH 7.4 @ 25°C
Reaction Conditions
- 1X Reaction Buffer
- 50 mM Tris-HCl
10 mM MgCl2
- 10mM (NH4)2SO4
- 4 mM DTT
- (pH7.5 at 25°C)
Inactivation
Unit Definition
One unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble material in 10 minutes at 30°C