Exonuclease III
Overview
Exonuclease III is a 3’→5’ exonuclease which acts by digesting one strand of a dsDNA duplex at a time or digesting the RNA strand of an RNA-DNA heteroduplex. Exonuclease III breaks phosphodiester bonds on the 5’ side of AP sites in both dsDNA and ssDNA and removes 3’ terminal groups on dsDNA, efficiently degrades 3’ recessed but not 3’ protruding DNA ends (creating 5’ overhangs)
Highlights
Catalyzes the linear or nicked dsDNA
Removal of nucleotides in the 3′ to 5′ direction
Product Source
Purified from a strain of E. coli that expresses the recombinant Exonuclease III gene
Product Usage
Supplied in
- 10 mM Tris-HCl
- 100 mM NaCl
- 0.1 mM EDTA
- 1 mM DTT
- 50% glycerol
- pH 7.4 @ 25°C
Reaction Conditions
- 10 mM Tris-HCl
- 10 mM MgCl2
- 1 mM DTT
- (pH 7 @ 25°C)
Inactivation
Unit Definition
One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble total nucleotide in 30 minutes at 37°C