Parallel capsids screening

Generation and screening of libraries of AAV variants have emerged as a powerful method for identifying novel capsids for gene therapy applications. For the majority of libraries, vast population diversity requires multiplexed production, in which a library of ITR-containing plasmid variants is transfected together into cells to generate the viral library. This process has the potential to be confounded by cross-packaging and mosaicism, in which particles are comprised of genomes and capsid monomers derived from different library members.

Parallel AAV platform is a recognized capsid library employed to methodically assess AAV serotypes or variations for diverse objectives with Barcode-seq technology. This platform comprises a single capsid variant being linked to a unique DNA barcode, which is more than 1000 distinct sequence. Through the comparative analysis of DNA or RNA levels associated with these barcodes, researchers can ascertain the AAV capsid variant that demonstrates the highest efficiency tailored to their particular application.  Parallel AAV platform could be used for second or third round selection of AAV library.