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Cuts dsDNA at a TelN recognition sequence (56 bp) and leaves covalently closed ends at the site of cleavage.
Catalog #: | Concentration | Size | Price (US) |
| 5,000 units/ml | 5,000 units | US$558 |
| 5,000 units/ml | 2,500 units | US$310 |
| 5,000 units/ml | 1,000 units | US$138 |
| 5,000 units/ml | 500 units | US$77 |
Highlights
Catalyzes dsDNA at a TelN recognition sequence (56 bp)
Leaves covalently closed ends at the site of cleavage
Product Source
Purified from a strain of E. coli that expresses TelN Protelomerase of phage N15.
Product Usage
Supplied in
10 mM Tris-HCl
100 mM NaCl
0.1 mM EDTA
1 mM DTT
50% glycerol
pH 7.4 @ 25°C
Reaction Conditions
1X ThermoPol® Reaction Buffer
20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.1% Triton® X-100
(pH 8.8 @ 25°C)
Inactivation
75°C for 5 minutes
Unit Definition
One unit is defined as the amount of enzyme required to cleave 0.5 μg of linearized control plasmid in a total reaction volume of 50 μl in 30 minutes at 30°C in 1X ThermoPol Reaction Buffer.
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